Nilaparavata lugens stal genetic divergence analysis. Delphacidae is a planthopper species that feeds on rice plants oryza sativa l. A nicotinic acetylcholine receptor mutation conferring target. The genetic inheritance and physiological effect of the eye color mutation in the bph have been studied, but the location of. Biological notes and effect of insecticides on the water measurer bug, hydrometra sp. One end of the egg is united near the egg caps, the other remains free. The objective of this study was to determine the susceptibility of n. An nilaparvata lugens in nahilalakip ha genus nga nilaparvata, ngan familia nga delphacidae. The delphacid genus nilaparvata consists of 18 species.
We studied the effects of n, p, and k application on chemical composition of rice plants and its consequences on life parametersadult longevity, fecundity, and egg hatchability of bph. In the present study, we further examined the transcriptome of n. The coccinellid micraspis crocea mulsant feeds on plants and on leafhoppers and other small insect pests of rice. Delphacidae, susceptibility of the treated rice plants and amounts of free amino acids and sucrose were studied. All structured data from the file and property namespaces is available under the creative commons cc0 license. Nilaparvata lugens elevated co 2 biochemical substances control ef. The migration of nilaparvata lugens stal delphacidae and other hemiptera associated with rice during the dry season in the philippines. Glutathione stransferases as antioxidant defence agents. The virus was found in the cytoplasm of the insect cells, sometimes associated with tubular structures, which is one of the characteristic features. Resistant rice varieties of a field nilaparvata lugens stal hemiptera. Nilaparvata is a crucial genus economically, with one amongst its member n. Biochemical analysis and synergistic studies with metabolic inhibitors indicated that elevated glutathione stransferases gsts with a predominant peroxidase activity conferred resistance to both pyrethroids. Nilaparvata lugens is one of the major pests of rice and results in substantial yield loss every year.
The genome of nlrv consists of 28 699 nucleotides and contains at least 11 large open reading frames orfs. We evaluated the parents and mapping population with six tests that measure varying combinations of the three. A neutral ceramidase, nlncdase, is involved in the stress. A nicotinic acetylcholine receptor mutation conferring. Paris of rice varieties carrying the bph1, bph2 and bph3 resistance genes are compared as hosts for n. Ecological fitness of brown planthopper, nilaparvata lugens stal, to rice nutrient management md. Phloem sap samples of resistant and susceptible rice plants were screened for uv lightabsorbing compounds by means of high performance liquid chromatography hplc. Effects of triazophos on biochemical substances of. The brown planthopper bph, nilaparvata lugens, sugar transporter gene 6 nlst6 is a facilitative glucosefructose transporter often called a passive carrier expressed in midgut that mediates sugar transport from the midgut lumen to hemolymph. A series of laboratory experiments was conducted using electrical penetration graph, salivary flange, and honeydew measurement to study the effects of feedinginduced intra and interspecific interactions on feeding behavior and honeydew excretion between planthoppers nilaparvata lugens stal and sogatela furcifera horvath. Aug 01, 2006 nilaparvata lugens adults were collected from insecticide free rice fields located on farms of zhejiang university. Files are available under licenses specified on their description page. The brown planthopper bph, nilaparvata lugens, is the most destructive insect pest in rice production worldwide.
Delphacidae population free from insecticides in the laboratory dong biqin1, 2, yang yajun1, xu hongxing1, zheng xusong1, k. Ecological fitness of brown planthopper, nilaparvata. The brown planthopper bph nilaparvata lugens is an economically important pest of rice in asia. Pymetrozine activates trpv channels of brown planthopper. Response of adult brown planthopper nilaparvata lugens. Ecological fitness of brown planthopper, nilaparvata lugens stal, to. The brown planthopper bph, nilaparvata lugens stal hemiptera. Here we combined 2de comparative proteomic and rnaseq transcriptomic analyses to identify fecundityrelated proteins and genes. The brown planthopper nilaparvata lugens stal, the most destructive pest of rice, has been identified, including biotypes with high virulence towards previously resistant rice varieties. Ecological fitness of brown planthopper, nilaparvata lugens. We used a mapping population of 1 doubledhaploid lines, produced from a cross between an improved indica rice variety ir64 and a traditional japonica variety azucena, to detect quantitative trait loci qtls for resistance to the brown planthopper bph, nilaparvata lugens.
Insects free fulltext transcriptomic analysis of the. Nilaparvata lugens an overview sciencedirect topics. The biochemical basis of resistance of rice plants to the brown planthopper, nilaparvata lugens, was investigated. Neonicotinoids, such as imidacloprid, are nicotinic acetylcholine receptor nachr agonists with potent insecticidal activity. The free yls in the hemolymph then move to the ovarioles near the pedicel. Bacterial symbionts of the brown planthopper, nilaparvata. Although nilaparvata seminula should be reexamined, 14 determined and 2 undetermined species are known as the members of the genus nilaparvata. Parasitic activities of egg parasitoids on the rice planthoppers. The nymphs showed an obvious moulting defect phenotype, lower survival rate and significantly reduced chitin content after mir.
Since its introduction in the early 1990s, imidacloprid has become one of the most extensively used insecticides for both crop protection and animal health applications. Resistance to nilaparvata lugens in rice lines introgressed with the. A serious pest of paddy, the brown planthopper nilaparvata lugens. Susceptibility to insecticides and ecological fitness in. The virus was found in the cytoplasm of the insect cells, sometimes associated with tubular structures, which is one of the characteristic features in tissues infected with reoviruses. Silencing a sugar transporter gene reduces growth and. In this study, we examined the bacterial microbes in n. Jan 18, 2018 the brown planthopper bph, nilaparvata lugens stal, is the most economically important rice pest throughout asian countries. Pdf rice viruses transmitted by the brown planthopper. Screening and functional analyses of nilaparvata lugens. Feedinginduced interactions between two rice planthoppers. Delphacidae is a major rice pest that harbors an endosymbiont ascomycete fungus, entomomyces delphacidicola str.
The brown planthopper bph, nilaparvata lugens is a devastating rice insect. In this study, our purpose was to reveal the components and functions of saliva in a rice sapsucking insect pest, nilaparvata lugens. Heong3, lu zhongxian1 1state key laboratory breeding base for zhejiang sustainable pest and disease control, institute of plant protection and. Most phloemfeeding insects secrete gelling and watery saliva during the feeding process. The color of compound eyes is an important biological characteristic of insects. Beetles are abundant in ricefields and could help suppress populations of several insect pests. In contrast, the brown plant hopper nilaparvata lugens uses a different mechanism to overcome sieve tube callose. Murugesan department of agricultural entomology, agricultural college and research institute, killikulam, vallanad628 252, india. The complete mitochondrial genome of nilaparvata lugens stal, 1854 captured in korea hemiptera. It also has been observed feeding on rice flower pollen. Our previous study found that the entomopathogenic fungus metarhizium anisopliae showed effective potential for controlling this pest. Nilaparvata lugens adults were collected from insecticide free rice fields located on farms of zhejiang university.
The complete mitochondrial genome of nilaparvata lugens. The influence of down regulating expression of sugar transporter genes on insect growth, development, and fecundity is unknown. The influence of down regulating expression of sugar transporter genes on insect growth. In the laboratory, adults were released in drum cages 20 cm diameter, 75 cm long containing booting plants of kmd1, kmd2, or nontransgenic xs11, and maintained in the chamber as described above. Oct 02, 20 as an rstrategy insect species, the brown planthopper bph nilaparvata lugens stal is a serious pest of rice crops in the temperate and tropical regions of asia and australia, which may be due to its robust fecundity. Pdf the brown planthopper bph nilaparvata lugens transmits both rice grassy. There have also been many reports of a yeastlike symbiont of n.
Selection of a laboratory colony of the brown planthopper nilaparvata lugens with the pyrethroids permethrin and. As with other classes of insecticides, resistance to neonicotinoids is a significant threat and has. Studies on host plant resistance to brown planthopper nilaparvata lugens stal. Nov 19, 2019 biological notes and effect of insecticides on the water measurer bug, hydrometra sp. Delphacidae, is an economically important insect of rice in asia heinrich, 1994. Feeding responses of the brown planthopper, nilaparvata. Experiments indicated that the effects of the tested pesticides were dependent on nymphal age, pesticide and their dose and time after application. Nilaparvata lugens is generally called as brown plant hopper or asian rice brown plant hopper bph. We evaluated the parents and mapping population with six tests that measure varying combinations of the.
Jun 14, 2005 neonicotinoids, such as imidacloprid, are nicotinic acetylcholine receptor nachr agonists with potent insecticidal activity. Insectfree rice plants were used in the following tests at the. However, the functions of salivary proteins are poorly understood. Media in category nilaparvata lugens the following 2 files are in this category, out of 2 total. An nilaparvata lugens in uska species han insecta nga syahan ginhulagway ni stsl hadton 1854. Biochemical bases of resistance to brown planthopper nilaparvata lugens stal in different rice accessions k. Effects of triazophos on biochemical substances of transgenic.
We studied the effects of n, p, and k application on chemical composition of rice plants and its consequences on life parametersadult longevity, fecundity, and egg. As with other classes of insecticides, resistance to neonicotinoids is a significant threat. Jun 25, 2001 selection of a laboratory colony of the brown planthopper nilaparvata lugens with the pyrethroids permethrin and. Buprofezin susceptibility survey, resistance selection and. Susceptibility to neonicotinoids and risk of resistance. Reference genes for quantitative realtime pcr analysis in.
Biochemical bases of resistance to brown planthopper. Adaptation of the brown planthopper, nilaparvata lugens stal, to. Antibiosis effects of rice carrying bph14 and bph15 on the. Bph causes significant damage to the rice crop by sucking sap from the xylem and phloem tissues, which ultimately leads to hopperburn and. Chromosomal location of a recessive redeye mutant gene in. Pdf taxonomy and biology of nilaparvata lugens hom. Effects of cry1ab toxin on propylea japonica thunberg. Seasonal abundance of macropterous nilaparvata lugens and. A reovirus in the brown planthopper, nilaparvata lugens. The accomplishment of the whole genome and transcriptome sequencing in n. Complete nucleotide sequence of the nilaparvata lugens.
Oct 10, 2019 among these insect pests, brown planthopper bph. However, little is known about the diversity of the symbiotic fungal ylss that are harbored in the eggs of bphs and the difference between fertilized eggs and nonfertilized eggs. Delphacidae, is one of the most harmful insect pests of rice plants, not only directly sucking the phloem sap but also transmitting diseases to host plants as a viral vector cuong et al. Buprofezin susceptibility survey, resistance selection and preliminary determination of the resistance mechanism in nilaparvata lugens homoptera. Response of adult brown planthopper nilaparvata lugens stal. The migration of nilaparvata lugens stal delphacidae and. Feedinginduced intra and interspecific interactions. Read seasonal abundance of macropterous nilaparvata lugens and sogatella furcifera based on presumptive macroptery in fifth. Its outbreaks constitute a serious threat to the sustainability of.
Detection and analysis of qtls for resistance to the brown. Nitrogen recycling in the brown planthopper, nilaparvata lugens. The nucleotide sequences of all genome segments of the nilaparvata lugens reovirus nlrv, which is found in the brown planthopper nilaparvata lugens, have been determined and some genes have been assigned to structural and functional proteins. Delphacidae, a serious insect pest of rice in tropical and temperate asia. The feeding responses, growth and population development of nilaparvata lugens stal are quantified on a range of twelve susceptible or resistant hostplants. In this study, the modified bulk seedling test method was used to identify the bph resistance level and host feeding choice of. Jiushuang zhang,1 wenhong li, 1dejiang dai, youwei lin, weijun zhou,1 and jinliang shen. Nilaparvata lugens, sogatella furczfera, egg parasitoid, anagrus, oligosita. Biochemical analysis and synergistic studies with metabolic inhibitors indicated that elevated glutathione stransferases gsts with a predominant peroxidase activity conferred resistance to both pyrethroids, whereas.
Pdf the complete mitochondrial genome of nilaparvata lugens. Results results indicated that substantial variations in the susceptibility to different insecticides. Blackwell publishing ltd dynamics of imidacloprid resistance. The planthopper transmits two kinds of viruses, rice ragged. Currently, chemical control is still a major method for suppressing n. Proteomic and transcriptomic analyses of fecundity in the. The brown planthopper nilaparvata lugens is one of the most serious rice plant pests in.